Friday, 15th February 25th Lorne Cancer Conference 2013

• Molecular Control of Mitochondrial Apoptosis by the BCL-2 Family - Emily Cheng
• Control of Apoptosis by the Bcl-2 Protein Family: Mechanisms and Relevance to Cancer Therapy - Jerry Adams
• Dead on Arrival:  How caspases promote and prevent cell death. - Douglas Green

• Senescence and melanoma: dissecting mechanism and screening for novel therapeutic targets - Daniel Peeper
• Finding and probing cancer drug targets using advanced in-vivo RNAi - Johannes Zuber
• GRHL3 functions as a tumour suppressor gene in HNSCC through enhanced stability of the proto-oncogene c-Myc - Stephen Jane
• Interrogating NUT Midline Carcinoma – an Aggressive and Fatal Cancer. - Alex H Beesley
• Mir-10b as a regulator of bone marrow mediated tumor angiogenesis - Albert S Mellick

“Using Microfluidic Engineering to Enable Physiologically Predicitive Cell Analysis”

In order to link knowledge of molecular mechanisms to phenotypes and disease states, the reductionist approach of cell biology has given way to the dynamic study of complex networks of interacting systems within single cells. Traditional, static 2D culture methods are often insufficient to provide relevant data for this type of experimentation. New microfluidic technologies for cell culture enable unprecedented control over the live cell environment. The key benefits of microfluidic cell culture are the creation of a more biologically relevant in vitro environment to predict in vivo activity and a precise, standardized format that is accessible to biologists. We have designed microfluidic cell culture chambers that recreate the mass transport environment of tissues, elicit cell responses to dynamic solution changes, model host-pathogen interactions, and enable long-term perfusion culture in a three-dimensional extracellular matrix. These design features were engineered into a standard format to facilitate liquid handling and live cell imaging. We incorporated a control system to automate the control of flow, temperature, and gas environment of the cells during experiments. This configuration allows the operation of application-specific microfluidic plates, including designs for media solution switching, spatial gradients, 3D culture, and single cell analysis. Our future work focuses on refining these physiologic models and expanding the set of application-specific designs.

Speaker:  Alex Mok, EMD Millipore

• IAPs, inflammation and cancer. - John Silke
• Small molecule inhibitors of the ubiquitin system - Mike Tyers

• Inhibiting protein protein interactions for cancer therapy  - David Lane

• Orphan Nuclear Receptor NUR77: A Potential Biomarker of Response to Epigenetic Treatment of Myelodysplasia - HongBin Liu
• Integrated genomics for prostate cancer - Geoff Macintyre
• Significant numbers of metabolically active irradiated tumour cells enter the circulation during a course of fractionated radiation therapy for non-small cell lung cancer: A novel finding with potential clinical significance - Michael P MacManus
• Proteomic analysis of 9 glioblastoma multiforme cell lines - Duthika Mallawaaratchy
• A systematic approach to identify novel cooperative tumour suppressor genes in Ras-driven tumourigenesis - Jan Manent
• The E3-ligase E6AP controls breast cancer invasion and motiliy by regulation of Rho-GTPases proteins - Mariam Mansour
• A synthetic-lethal screen for innate platinum resistance genes in lung adenocarcinoma - Kieren Marini
• Investigating the Inhibitory and Radiosensitising Properties of Dietary Polyphenols Resveratrol and [10]-Gingerol Against Metastatic Breast Tumour Cells - Ana Carolina Martin
• The relationship between Scribble structure, localisation and function - Claire A Martin
• Human epithelial ovarian cancer cell lines differentially express Sprouty proteins 1, 2 and 4 - Samar Masoumi Moghaddam
• Regulation of BH3-only proteins downstream of IL-3 and GM-CSF signalling - Dimitra Masouras
• Target gene identification of microRNAs associated with lymph node metastases in triple negative breast cancer - Andrea Mathe
• Reduced proliferation of murine B-cell lymphoma and acute myeloid leukaemia through RNAi-mediated loss of HDAC 3 occurs in a p53-dependent manner - Geoffrey Matthews
• TEMs (Tie2 Expressing Monocytes): Novel targets and effectors of anti-cancer therapies - Roberta Mazzieri
• Loss of the Tumor Suppressor Gene HIC1 Promotes Oncogenic Transformation in Mouse Embryonic Fibroblasts - Lisa McKenzie
• DNA Adduct-Forming Derivatives of the Anticancer Agents Mitoxantrone and Pixantrone - Jelena Medan
• Role of 2-morpholine substituted-1,3-benzoaxine family of compounds as chemo-sensitising agents, independent of DNA-PK - Terri Meehan-Andrews
• Phosphotyrosine profiling to define new therapeutic targets in infant MLL - Agnes Mendomo
• Cis-regulation of non-codings RNAs within the HOXC locus in breast cancer - Michael Milevskiy
• Role of APC in Mitochondrial Transport - Kate Mills
• Therapeutic and Research Potential of Stem cells - Stephen Minger
• Leptin increases the metastatic potential of breast cancer cells. - Ameet K. Mishra
• Plasma vitronectin predicts response to fluoropyrimidine-oxaliplatin chemotherapy in metastatic colorectal cancer - Mark P Molloy
• Cavin-1 alters oncogenic effects of caveolin-1 microdomains in prostate cancer - Hyeongsun Moon
• mRNA expression analysis of p53 isoforms in breast cancer - Brianna Morten
• Different protocols for Cryobiopsy versus Forceps Biopsy in Diagnosis of Patients with Endobronchial Tumors - amirhossein mozafarykhamseh
• Evaluation of anti-inflammatory kinase inhibitors for glioblastoma therapy - Lenka Munoz
• Identification and filtering of noisy probes for the Infinium HumanMethylation450 array - Haroon Naeem
• A Myc transcriptional program that is independent of EMT drives a poor prognosis tumour- propagating phenotype in HER2+ breast cancer - Radhika Nair
• Loss of ATRX or DAXX facilitates cellular immortalisation via the Alternative Lengthening of Telomeres pathway - Christine E Napier
• Functional characterization of microRNA isoforms with differential 3’ends - Corine T Neilsen
• The mutant p53 secretome drives invasion - Paul M Neilsen
• Elucidating the mechanisms of gene inactivation in a murine p53 shRNA-based Osteosarcoma model - Alvin JM Ng
• New roles for Interleukin-22 in the promotion of inflammation-associated gastric cancer.  - Paul Nguyen
• L1 Retrotransposition and Somatic Genomic Instability in High-Grade Serous Ovarian Cancer   - Thu H M Nguyen
• Exploiting the receptor uPARAP/Endo180 for targeted delivery of anti-cancer drugs to sarcomas and glioblastomas - Christoffer Nielsen
• Sex steroid receptor signalling and immune function - Rebecca Nightingale
• High expression of the telomerase-RNA binding protein dyskerin contributes to the proliferation and survival of neuroblastoma cells and is predictive of poor patient outcome - Rosemary O'Brien
• Hck Drives Tumour Associated Macrophage Transcriptional Profiles in Myeloid Cells Promoting Pulmonary Inflammation and Cancer. - Robert JJ O'Donoghue
• Kinetic modelling and the intrinsic apoptosis pathway - Martin O'Hely
• Jersey regulates mammary development and plays a key role in human breast carcinogenesis. - Samantha R Oakes
• Epigenomes of mouse mammary epithelial subsets - Bhupinder Pal
• Understanding Oncogenic PI3K in Colorectal Cancer - Michelle Palmieri
• Phosphoproteomics of cellular response to Vemurafenib reveals reprogramming by oncogenic B-RAF protein in Thyroid Cancer - Rob Parker
• A Bifunctional Fluorescent Assay for the Assessment of Cytotoxicity and Proliferation - Rachael Parry
• Molecular Changes Associated with Transformation of Liver Progenitor Cells - Adam Passman
• A telomere protective function of hTERT that is independent of catalytic activity. - Omesha Perera
• Therapeutic Approach to Target Mesothelioma Cancer Cells Using the Wnt Antagonist sFRP4: Metabolic State Of Cancer Cells - Vanathi Perumal
• A bioinformatic pipeline for the analysis of miRNAs in the PMC42 and MDA-MB-468 model systems of human breast cancer. - Gayle K Philip
• Identification and functional characterisation of miRNAs in breast development and malignancy - Yuwei Phua
• Variant repeats are interspersed throughout the telomeres of ALT cells, resulting in altered telomeric architecture - Hilda A Pickett
• A Single Transcriptional Program Underpins Patient Survival in Pancreatic Cancer - Mark Pinese
• An in vitro assay to screen novel potential driver genes of pancreatic carcinogenesis - Andreia V. Pinho
• Understanding Cancer Cell Populations in Breast Cancer – A Functional Genomics Approach - Cletus A Pinto
• Targeting a Novel MicroRNA Angiogenesis Pathway as a Next Generation Cancer Therapy - Prue N Plummer
• Haematopoietic Cell Kinase Promotes the Development and Progression of Colorectal Cancer in Mice. - Ashleigh Poh
• SOX11 as a potential target of FUS-DDIT3 in Myxoid Liposarcoma - Sophie Popkiss
• A DISINTEGRIN AND METALLOPROTEINASE ADAM-9 REGULATES THE METASTATIC PROPERTIES OF BREAST TUMOUR CELLS - Normand Pouliot
• Investigating modes of action for Pixantrone cytotoxicity and lack of cardiotoxicity - Paul P Pumuye
• Cytokines as key targets in cancer related inflammation  - Tracy Putoczki
• A Novel Inhibitor of Ribosomal RNA Gene Transcription Identifies both p53-Dependent and p53-Independent Checkpoint Responses to Perturbation of Pol I Transcription - Jaclyn Quin
• Genomic analysis of human brain metastases: an integrative approach     - Michael C J Quinn†
• RADIOSENSITIZING EFFECT OF BENZ 1, 3-OXAZINES AS NOVEL DNA-PK INHIBITORS IN THE TREATMENT OF CANCER - Suraj Radhamani
• Deconstructing the invasive machinery of cancer cells. - Troy J Ralph
• An siRNA screen to identify novel regulators of the cell cycle G₂/M transition. - Max V. Ranall
• The anti-angiogenic properties of sulfated β-cyclodextrins in anti-cancer formulations incorporating 5-fluorouracil - Marie Ranson
• Stimulating the innate type-1 interferon pathway reduces breast cancer metastasis to bone - Jai Rautela
• Shifting doxorubicin activity from topoisomerase2 poison to adduct formation reduces cardiotoxicity while maintaining anticancer efficacy - David M Rayner
• Can cell death drive TNF dependent skin inflammation? - James Rickard
•  PRIOR TO THE ONSET OF ADVANCED DISEASE, CANCER REPOPULATING CELLS  IN LOCALISED PROSTATE TUMOURS ARE CASTRATE-RESISTANT AND STEM-LIKE. - Gail Risbridger
• CAPTIV: The Cancer of Prostate Translational Research in Victoria Collaboration - Gail P Risbridger
• Identification of new BIM regulators using over-expression and knockdown libraries - Leona Rohrbeck
• Sirtuin 1 is a novel regulator of pancreatic carcinogenesis   - Ilse Rooman
• Combination therapy with raloxifene and a novel curcumin analog for the treatment of hormone refractory prostate cancer: in vitro mechanisms for synergistic cytotoxicity - Rhonda J Rosengren
• Next-Generation Sequence Analysis of Cancer Xenografts - Fernando Rossello
• Genomic profiling of primary mucinous ovarian carcinomas - Georgie L Ryland
• Differentiation Therapy for t(8;21) Acute Myeloid Leukemia - Jessica Salmon
• A novel therapeutic target in high-grade serous ovarian cancer: Connective Tissue Growth Factor (CTGF) - Goli Samimi
• Bioenergetic modulation sensitises acute lymphoblastic leukaemia to glucocorticoids - Amy L Samuels
• Identification of microRNAs that function downstream of Brca1 - Gurveen Sandhu
• Chemokine signalling in breast cancer angiogenesis - Michael J Sax
• Sex steroid receptor signalling in Flu specific T cells. - Manvendra Saxena
• Formation of an immunosuppressive pre-metastatic niche in breast cancer triggered by hypoxic tumour-derived factors - Jaclyn Sceneay
• Investigating DNA methylation in paediatric central nervous system tumours - Alexandra Sexton-Oates
• Histone deacetylase 2 promotes N-Myc oncogenesis by repressing the transcription of p53 induced nuclear protein 1 - Jeyran Shahabzi
• Defining Ubiquitin Ligase Function in Breast Cancer - Robert Shearer
• Mutagenic potential of direct apoptosis inducers - Tanmay Shekhar
• Targeting MUC13 to sensitise colorectal cancer cells to apoptosis - Yong H Sheng
• Evaluation of the CDK4 Inhibitor PD-0332991 in Melanoma and Identification of Genomic Predictors of Sensitivity - Karen E Sheppard
• Hhex regulates therapeutic resistance of Lmo2-induced pre-Cancerous Stem Cells. - Benjamin J Shields
• RNA aptamers targeting CD133 as a novel targeted therapeutic for the treatment of cancer - Sarah L Shigdar
• Regulation of the matricellular proteins CCN1/Cyr61 and CCN2/CTGF by vitronectin induces cancer cell survival - Ali Shokoohmand
• Screen small, diverse compound subsets from the Queensland Compound Library “Open” collection - Moana M Simpson
• A novel role for pericytes as ‘cancer associated fibroblasts’ in ovarian cancer progression and metastasis  - Devbarna Sinha
• A ROLE FOR THE FREE BETA SUBUNIT OF HUMAN CHORIONIC GONADOTROPIN IN SENSITIVITY OF EPITHELIAL OVARIAN CANCER CELLS TO PLATINUM-BASED CHEMOTHERAPEUTICS - Snega S Sinnappan
• DNA Damage Response in Peripheral Blood Lymphocytes and Out-of-Field Hair Follicles During Radiotherapy of Patients with Non-Small Cell Lung Cancer: A Prospective Clinical Trial - Shankar Siva
• GENOME-WIDE GAIN-OF-FUNCTION SCREEN FOR GENES THAT INDUCE EPITHELIAL TO MESENCHYMAL TRANSITION IN BREAST CANCER - Dubravka Skalamera
• Generation of a Genome Scale Lentiviral Vector Library for EF1α Promoter- Driven Expression of Human ORFs and Identification of Human Genes Affecting Viral Titer - Dubravka Škalamera
• Neural regulation of metastasis: New avenues for therapeutic intervention - Erica K Sloan
• Inactivation of USP44, a regulator of global H2BK120ub levels, is an early event in the formation of colorectal cancer - Mathew A. Sloane
• Tumour Suppression via Re-equilibration of Cell Polarity Networks. - Lorey Smith
• Discovery of microRNAs associated with Breast Cancer Epithelial Mesenchymal Plasticity using Bioinformatics and Next Generation Sequencing - Eliza Soo
• Flavopiridol – a novel therapeutic option for NUT midline carcinoma - Anja Stirnweiss
• BCL-2 expression is silenced by DNA hypermethylation in human breast cancer, providing a potential biomarker of sensitivity to mitotic inhibition. - Andrew Stone
• The loss of Cdkn2a locus is a critical event in the transformation of liver progenitor cells - Robyn Strauss
• Deletion of Myc limits the growth of Stat3-driven gastric tumours - Emma C Stuart
• Functional analysis of TERT locus polymorphisms which are associated with risk of breast and ovarian cancer - Michael D Stutz
• Histone deacetylase 5 forms a transcriptional repressor complex with N-Myc and blocks tumour cell differentiation - Yuting Sun
• CHARACTERIZATION OF NOVEL DNA-PK INHIBITOR FROM ISOFORM SPECIFIC PI3K AS AN ANTI-CANCER DRUG - Rejitha Suraj
• High-throughput identification and characterization of novel enhancer molecule for hydroxamate-based histone deacetylase inhibitors - Aldwin Suryo Rahmanto
• Detection of autophagy - AlphaLISA assays - Margareta Sutija
• Metastatic tumours promote monocyte mobilisation - Agnieszka Swierczak
•   Role of the unfolded protein response in melanoma progression and metastasis - Erin K Sykes
• Loss of Tumor Suppressor Hic1 Accelerates the Progression of Lung Adenocarcinoma Induced by Oncogenic KRas - Anette Szczepny
• Molecular analysis of circulating (CTC) and disseminated tumour cells (DTC) in human breast cancer xenograft models. - Anthony Tachtsidis
• The atypical kinase SgK223 is a novel mediator of cell invasion in pancreatic cancer - Carole M Tactacan
• Frequent alterations in mTOR signaling in human ovarian serous carcinomas - Pradeep Tanwar
• Assessing the regulatory function of small RNAs - Daniel W Thomson
• Multiple mechanisms of resistance to targeted inhibitors in c-Kit mutant melanoma - Jason Todd
• Evaluation of the efficacy of bromodomain inhibitors for the treatment of colorectal cancer - Lars Tögel
• Differential Effects of Oxytocin on Androgen Independent Prostate Cancer Cells - Nicole C Tom
• A FUNCTIONAL IN VIVO shRNA SCREEN FOR BREAST CANCER METASTASIS SUPRESSOR GENES - Rebeka Tomasin
• Identification and validation of two novel metastatic markers in Osteosarcoma. - Eleni Topkas
• Designing targeted therapies for High-Grade Serous Ovarian Cancer (HG-SOC) utilising a novel xenograft cohort stratified according to molecular defects - Monique D Topp
• Absence of senescence traits in human melanocytic nevi - Sieu L Tran
• Histone deacetylase and proteasome inhibitors induce overlapping transcriptional responses and synergistically induce apoptosis in tumour cells. - Janson JT Tse
• The antimicrobial peptide LL-37 inhibits migration of prostate cancer cells - Yan Tu
• Complete loss of the p53 effectors p21, Puma and Noxa does not predispose mice to spontaneous tumor development     - Liz J Valente
• Understanding the role of tumour suppressor TRIM35 in TGF-ß signaling - Joanne H. van Vuuren
• A new Bcl-2-specific BH3-mimetic has in vivo efficacy without provoking thrombocytopenia - Cassandra Vandenberg
• Investigating the role of exosomes in promoting melanoma metastasis. - Laura J Vella
• Combined targeting of JAK2 and Bcl-2/Bcl-xL as a novel, potentially curative treatment for haematological malignancies expressing mutant JAK2 - Michaela Waibel
• High-grade mucinous ovarian cancer: from where does it originate and how should it be treated? - Matthew Wakefield
• MYD88 mutation in B-cells - effects on NFkB activity, B-cell survival and proliferation - James Q Wang
• STAT3 inhibitor S3I-201 suppresses human osteosarcoma cell growth - Xiaochun Wang
• Anti-cancer efficacy of histone deacetylase inhibitors mediated by immune system - Alison C West
• Identifying predictors of sensitivity to PF-00477736 in melanoma - Britanto Wicaksono
• Dynamic expression of EMT-related mRNA alternative splicing variants in human mammary carcinoma - Edwin Widodo
• Not so minor after all. Emerging roles for “minor class” splicing in cancer. - Benjamin B Williams
• In Vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer  - Louisa CE Windus
• Defining the gene regulatory network controlled by the tumour suppressor Ikaros in thymocytes and T cell leukemia using dynamic in vivo RNAi - Matthew Witkowski
• Understanding the mechanism of erythroid enucleation: a chemical genetics approach - Christina B Woelwer
• E6-Associated Protein (E6AP) regulates the INK4/ARF locus and oncogene-induced senescence - Kamil Wolyniec
•  Achieving increased chemotherapeutic efficacy in breast cancer by loss of Siah2 - Christina S.F. Wong
• Increased Interferon Response Gene Expression During Megakaryocyte Ontogeny: Implications for the Spontaneous Resolution of Down Syndrome Transient Myeloproliferative Disorder - Andrew J. Woo
• Novel small molecules that target the dimer interface of 14-3-3 proteins as a basis for possible anti-cancer therapeutics. - Joanna Woodcock
• Processing and presentation of NY-ESO-1 epitopes by melanoma cells. - Katherine Woods
• Isolation and characterization of prostate cancer initiating cells based on aldehyde dehydrogenase activity - Jinhan (Angela) Xie
• mTOR Inhibition in Biliary Tract Cancer - Yvonne Yeung
• Fibroblast Growth Factor Receptor 1 Gene Amplification in Oral Tongue Squamous Cell Carcinoma - Frequency and Associations with Clinical Features and Patient Outcome - Richard J Young
• Transcription Factor ATF3 Suppresses Bladder Cancer Metastases by Regulating Gelsolin-Mediated Actin Cytoskeleton Remodeling - Liang Yu
• Characterization of the novel broad-spectrum kinase inhibitor CTx-0294885 as an affinity reagent for mass spectrometry-based kinome profiling - Luxi Zhang
• Human Breast Cancer Cell Lines and micro-RNAs in the Quail Embryo – A model for short term in vivo responses. - Tania Zheng
• Genome-wide DNA methylation analysis of archival formalin-fixed paraffin-embedded tissue (FFPET) using MBDCap-Seq identifies novel epigenetic diagnostic biomarker loci in breast cancer  - Elena Zotenko*
• The biological function of parallel telomeric G-quadruplexes and their interaction with the cancer associated enzyme telomerase - Katherine G Zyner
• Triazolophanes as a novel class of G-quadruplex binding agents - Lucy Ping
• The inflammation-resolving trihydroxy-docosahexaenoic acid derivative, resolvin D2 supports MCF-7 cell proliferation via activation of estrogen receptor - Nuha Alzaubai
• The prognostic significance of ALDEHYDE DEHYDROGENASE 1 (ALDH1) and CD133 expression in early-stage non-small cell lung cancer. - Alamgeer Muhammad